Are there any substances that are incompatible with bead based multiplex assays?

  • Heparin. Should not exceed 10 IU per mL of sample. It can cause bead agglutination and increased signals.
  • DMSO and other similar organic solvents. May cause the leaching of dye from the beads making sample analyte levels undetectable.
  • SDS and other similar denaturing agents. Should not exceed 0.1% in sample. These reagents can cause improper capturing of analytes.