Human Angiogenesis Array & Growth Factor 17-plex Array (HDAGP17)

We Will Test Your Sample(s) and Provide Results For The Following Biomarkers: Angiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1, FGF-2, Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PlGF, VEGF-A, VEGF-C, VEGF-D

Discovery Price $26.19/sample (single analysis / includes sample filtration)

Single analysis Min. volume required: 50 uL, Preferred volume: 75 uL

Duplicate analysis Min. volume required: 75 uL, Preferred volume: 100 uL

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel)

No
Yes

(Category A)

Yes

(Category B)

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SKU: HDAGP17 Category: Tag:

Description

Biomarker Analytes:

Angiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1, FGF-2, Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PLGF, VEGF-A, VEGF-C, VEGF-D.

Price:

$26.19 / sample (single analysis)

Volume Required:

Serum & Plasma: 30μL for single, 45μL duplicate, 60μL triplicate analysis.

Other sample types: 50μL single analysis. 75μL duplicate analysis. 100μL triplicate analysis.

Biomarker Research Areas:

Vascular Development, Cancer, Cardiovascular Diseases, Diabetic Ulcers, Macular Degeneration, and Wound Healing.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Human Angiogenesis / Growth Factor Array facilitates research on vascular development, cancer, cardiovascular diseases, diabetic ulcers, macular degeneration, and wound healing. This Discovery Assay® measures 17 angiogenesis / growth factor biomarkers simultaneously in a single microwell: Angiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1, FGF-2, Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PLGF, VEGF-A, VEGF-C, VEGF-D. The assay is designed for protein analysis in serum, plasma, cell cultures, and tissue homogenates. Other sample types can be tested, however we recommend a pilot run.

Background Information:

Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration and cancer. Angiogenesis plays a significant role in tumor growth and metastasis.

In tumors, dysregulated signalling and hypoxic conditions lead to sustained, almost uncontrolled angiogenesis – a necessary component of tumor growth and metastasis. Chronic inflammation mechanisms, such as the production of reactive oxygen species during infection and secretion of pro-inflammatory cytokines can also foster angiogenesis in tumor progression. Angiogenic signalling in tumors is similar to normal angiogenesis, mediated by soluble growth factors, membrane-bound receptors, and cell-cell and cell-matrix interactions. Such signalling

regulates cell migration, which is vital to angiogenesis. However, there are multiple differences between tumor angiogenesis and normal blood vessel formation. Tumor endothelial cells proliferate faster than non-tumor endothelial cells. Tumor vasculature differs from normal vasculature in morphology, enhanced leakiness, and structural abnormalities. Finally, tumor vessels are often not capable of transporting oxygen to and removing waste products from all of the tumor tissues, resulting in frequent tumor cell necrosis.

Intended Sample Type:

Human serum or plasma (EDTA recommended), cell cultures, tissue homogenates and others. A pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

Serum and Plasma samples will be diluted 1:3 by Eve Tech to bring protein concentrations within the standard curve range.

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Multiplex Immunoassay analyzed with a BioPlex 200

Angiogenesis Array Assay Kit Source:

Millipore MILLIPLEX

 

Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay* Inter-assay* Accuracy
pg/mL %CV %CV % Recovery
Angiopoietin-2 3.0 3.0 10.3 71
BMP-9 1.2 4.6 7.5 107
EGF 1.0 3.2 6.8 83
Endoglin 17.0 4.1 6.4 85
Endothelin-1 1.4 2.6 5.0 131
FGF-1 4.6 4.4 6.4 86
FGF-2 8.6 2.8 7.1 88
Follistatin 11.1 3.2 9.8 42
G-CSF 5.4 4.4 8.1 89
HB-EGF 0.4 3.8 13.1 96
HGF 8.5 4.3 10.7 81
IL-8 0.2 3.0 7.1 86
Leptin 42.8 2.8 6.0 79
PLGF 0.7 4.0 6.9 74
VEGF-A 8.1 3.5 10.0 99
VEGF-C 2.2 4.2 6.3 77
VEGF-D 1.9 3.4 7.1 130

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Got something to discuss?


Customer
luba.perry
8 months 1 day ago

Hello,
Can I test both human angiogenesis array together with rat albumin? meaning from the same sample? or I have to prepare 2 different tubes for that?
Also, can a 1:5 dilution of mouse serum be okay for the test? I don’t have a large volume of the samples.
Thanks!

 
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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