Description

Biomarker Analytes:

sCD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A

Price:$54.99/ sample (single analysis)
Volume Required:
50μL single analysis. 75μL duplicate analysis. 100μL triplicate analysis.

Biomarker Research Areas:
Immune response, inflammation, allergy, irritable bowel disease (IBD), sepsis, and cancer.
Multiplex Assay Overview:
The assay service provided by Eve Technologies on their Human Cytokine Array / Chemokine Array 48-Plex facilitates research in any area that involves the immune system such as inflammation, allergies, irritable bowel disease (IBD), sepsis, and cancer. This Discovery Assay® measures forty-one biomarkers simultaneously in a single microwell. The assay is designed for extracellular protein analysis in nearly all types of biological samples.

Background Information:

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

Intended Sample Type:

Human serum, plasma (EDTA recommended), cell cultures, tissue homogenates, synovial fluid, CSF, sputum, BAL, urine and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.
Special Sample Preparation:

ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane.

Usage Statement:
Unless otherwise stated our products and services are intended for research use only.

Detection Method:
Multiplex Immunoassay analyzed with a BioPlex 200.

Cytokine Array Assay Kit Source:
Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy

*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.

% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Reference Ranges – 25th percentile to 75th percentile.

50% of samples tested have been observed to be within this range. 25% have been observed to be below this range and 25% have been observed to be above this range.

*Values generated from undiluted samples. RANTES may need to be analyzed with a dilution factor due to high concentrations in serum and plasma. Eve Technologies’ “Human Supplemental Biomarker Array 10-plex” Discovery Assay Includes these targets and is run at a 1:100 dilution. It is recommended to use this panel or a custom assay to generate accurate results for these biomarkers.