* NEW – Human Cytokine Array/Chemokine Array 71-Plex Panel (HD71)

We Will Test Your Sample(s) and Provide Results For The Following Biomarkers:

sCD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A, 6Ckine, BCA-1, CTACK, ENA-78, Eotaxin-2, Eotaxin-3, I-309, IL-16, IL-20, IL-21, IL-23, IL-28A, IL-33, LIF, MCP-2, MCP-4, MIP-1d, SCF, SDF-1A+β, TARC, TPO, TRAIL, TSLP.

** NOTE: Results will not directly correlate with the previous HD42 or HD65 assay, as a result of the antibody systems being optimized in this new panel.
If you are in need of the previous HD42 or HD65 assay, to correlate with past data, please contact us directly.

Discovery Price $79.99/sample (single analysis / includes sample filtration)

Single analysis volume required: 100uL, Duplicate analysis volume required: 150uL

Recommended Dilution:  *Note: RANTES has high concentrations in serum and plasma. If these targets are important to your study and your sample type is serum or plasma, it is recommended to run these 3 targets in a separate assay with a dilution factor applied or utilize our “Human Supplemental Biomarker Array” (HDHSB10).  Note we also have limited wells in the Featured 3-plex, HPDGR-03-09.

****** If you expect your samples to have low cytokine levels (i.e. normal human population studies), inquire about a High Sensitivity panel (HDHSTC14).

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
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Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
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(1 Part Sample and 1 Part Diluent)

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0.5-0.65mL Snap Cap

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1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

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Other

(Non Compatible Tubes $3.00/ Tube)

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(ie. S1,S2,S3)

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Description

Biomarker Analytes:

sCD40L, EGF, Eotaxin, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, MDC (CCL22), MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGFα, TNFα, TNFβ, VEGF-A, 6Ckine, BCA-1, CTACK, ENA-78, Eotaxin-2, Eotaxin-3, I-309, IL-16, IL-20, IL-21, IL-23, IL-28A, IL-33, LIF, MCP-2, MCP-4, MIP-1d, SCF, SDF-1A+β, TARC, TPO, TRAIL, TSLP

Price:

$79.99/ sample (single analysis)

Volume Required:

100μL single analysis. 150μL duplicate analysis.

Biomarker Research Areas:

Immune response, inflammation, allergy, irritable bowel disease (IBD), sepsis, and cancer.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Human Cytokine Array / Chemokine Array 71-Plex facilitates research in any area that involves the immune system such as inflammation, allergies, irritable bowel disease (IBD), sepsis, and cancer. This Discovery Assay® measures forty-one biomarkers simultaneously in a single microwell and twenty-three biomarkers simultaneously in another microwell. The assay is designed for extracellular protein analysis in nearly all types of biological samples.

Background Information:

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such

as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

Intended Sample Type:

Human serum, plasma (EDTA recommended), cell cultures, tissue homogenates, synovial fluid, CSF, sputum, BAL, urine and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane.

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Multiplex Immunoassay analyzed with a BioPlex 200

Cytokine Array Assay Kit Source:

Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay Inter-assay Accuracy
Analyte pg/mL %CV %CV % Recovery
6CKine 45.4 8.6 13.7 93.3
BCA-1 1.3 4.8 6.2 101.3
CTACK 1.8 8.2 9.2 98.5
EGF 2.8 2.3 5.8 97.5
ENA-78 7.2 4.3 6.2 107.9
Eotaxin 4.0 7.2 10.8 100.5
Eotaxin-2 4.4 4.5 8.1 100.4
Eotaxin-3 8.7 8.3 14.5 104.2
FGF-2 7.6 2.3 4.8 99
Flt-3L 5.4 2.4 6.6 98.2
Fractalkine 22.7 4.5 9.4 87.2
G-CSF 1.8 1.8 15.5 100.3
GM-CSF 7.5 3.1 10.1 100.7
GRO 9.9 2.1 9.2 97.5
I-309 1.4 7.0 11 103.4
IFNa2 2.9 2.4 13.3 93.9
IFNɣ 0.8 1.6 12 98.1
IL-1α 9.4 3.3 12.8 92.9
IL-1β 0.8 2.3 6.7 94.9
IL-1ra 8.3 2.1 10.7 93.5
IL-2 1.0 2.1 6.3 95.4
IL-3 0.7 3.4 6.1 101
IL-4 4.5 2.9 14.2 94.5
IL-5 0.5 2.6 10.8 99.9
IL-6 0.9 2.0 18.3 96.1
IL-7 1.4 1.7 16.1 93
IL-8 0.4 1.9 3.5 98.3
IL-9 1.2 2.4 8.4 99.4
IL-10 1.1 1.6 16.8 97.7
IL-12 (p40) 7.4 2.8 12.4 93.3
IL-12 (p70) 0.6 2.2 16.7 104
IL-13 1.3 2.2 9.2 95
IL-15 1.2 2.7 8.1 95.3
IL-16 9.1 3.5 16.6 96.6
IL-17A 0.7 2.2 7.9 103.8
IL-18 0.4 3.9 4.6
IL-20 53.3 4.6 7.6 98.5
IL-21 6.8 5.6 12.7 99.8
IL-23 31.5 4.6 7.5 99.7
IL-28A 7.9 4.4 10.1 98.5
IL-33 6.2 5.2 7.2 103.9
IP-10 8.6 2.6 15.3 93.8
LIF 5.8 6.0 12.1 102
MCP-1 1.9 1.5 7.9 98.3
MCP-2 2.2 4.5 7.3 106.2
MCP-3 3.8 1.6 6.4 97
MCP-4 3.4 3.6 6.1 104.6
MDC 3.6 1.6 7.2 102.3
MIP-1d 10.5 5.3 12 94.1
MIP-1α 2.9 1.9 14.5 105
MIP-1β 3.0 2.4 8.8 92.4
PDGF-AA 0.4 4.3 16.7 97.9
PDGF-BB 2.2 2.1 12.3 102
RANTES 1.2 1.9 5 93.8
sCD40L 5.1 3.7 18.9 95.2
SCF 5.6 7.4 11.6 99.4
SDF-1α+β 55.8 11.1 9.2 81.3
TARC 0.4 5.6 9.8 100.9
TGF-α 0.8 4.1 9.5 91.7
TNFα 0.7 2.6 13 97.8
TNFβ 1.5 1.6 11.4 97.5
TPO 37.9 8.6 9.8 99.7
TRAIL 3.5 5.3 10.2 102.9
TSLP 3.1 6.9 13.8 109.5
VEGF-A 26.3 3.7 10.4 91.8

Reference Ranges
25th percentile to 75th percentile.
50% of samples tested have been observed to be within this range. 25% have been observed to be below this range and 25% have been observed to be above this range.

*Values generated from undiluted samples. RANTES may need to be analyzed with a dilution factor due to high concentrations in serum and plasma. Eve Technologies’ “Human Supplemental Biomarker Array 10-plex” Discovery Assay Includes these targets and is run at a 1:100 dilution. It is recommended to use this panel or a custom assay to generate accurate results for these biomarkers.

Analyte Serum (pg/mL) Plasma (pg/mL)
Percentile 25th 75th 25th 75th
6CKine TBD
BCA-1 TBD
CTACK TBD
EGF 18.5 104.2 6.2 58.1
ENA-78 TBD
Eotaxin 50.0 147.6 56.8 145.0
Eotaxin-2 TBD
Eotaxin-3 TBD
FGF-2 18.5 62.2 41.9 120.1
Flt-3L 0.02 25.7 0.01 66.39
Fractalkine 4.8 59.8 33.67 113.5
G-CSF 11.7 31.8 16.4 53.1
GM-CSF 2.8 70.5 7.1 44.1
GRO 474.9 1,273.1 288.7 734.04
I-309 TBD
IFNa2 5.4 22.3 10.5 69.0
IFNɣ 3.6 18.1 4.5 50.8
IL-1α 0.0 17.1 0.01 63.41
IL-1β 0.02 6.0 0.01 6.3
IL-1ra 1.6 38.4 35.7 165.8
IL-2 0.01 5.3 0.01 4.36
IL-3 0.01 0.9 0.01 0.84
IL-4 0.01 14.3 0.01 2.2
IL-5 0.2 1.0 0.2 0.9
IL-6 0.1 5.5 0.9 12.11
IL-7 1.7 5.0 0.53 2.3
IL-8 12.0 41.9 9.6 99.5
IL-9 0.0 2.4 0.2 2.4
IL-10 0.7 8.4 1.0 7.8
IL-12 (p40) 0.6 55.6 0.01 38.6
IL-12 (p70) 1.3 7.7 2.3 20.74
IL-13 0.3 6.4 0.01 4.58
IL-15 0.6 6.2 0.01 5.7
IL-16 TBD
IL-17A 0.9 16.9 3.5 40.4
IL-20 TBD
IL-21 TBD
IL-23 TBD
IL-28A TBD
IL-33 TBD
IP-10 76.8 185.6 204.4 766.9
LIF TBD
MCP-1 289.7 611.8 184.0 389.8
MCP-2 TBD
MCP-3 0.7 28.5 0.01 32.87
MCP-4 TBD
MDC 872.2 1,978.3 592.1 1,390.3
MIP-1d TBD
MIP-1α 0.02 14.4 1.87 16.43
MIP-1β 15.2 47.1 23.78 91.17
PDGF-AA* 1,055.3 3,951.5 143.1 730.7
PDGF-BB* 4,588.5 9,532.8 1,025.1 3,877.2
RANTES* 941.8 2,311.3 935.3 33,600.8
sCD40L 1,829.7 8,638.1 311.7 2,225.0
SCF TBD
SDF-1 TBD
TARC TBD
TGF-α 2.0 7.5 0.9 4.7
TNFα 4.3 17.0 8.4 23.0
TNFβ 0.0 9.5 0.01 6.4
TPO TBD
TRAIL TBD
TSLP TBD
VEGF-A 41.8 220.9 48.7 215.7

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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