Human High Sensitivity T-Cell Discovery Array 14-plex (HDHSTC14)

We Will Test Your Sample(s) and Provide Results For The Following Biomarkers:

GM-CSF, IFNy, IL-1B, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-23, TNF-a

Discovery Price $26.69/sample (single analysis / includes sample filtration)

Single analysis volume required: 50uL, Duplicate analysis volume required: 75uL

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

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(Category A)

Yes

(Category B)

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SKU: HDHSTC14 Category: Tag:

Description

Biomarker Analytes: GM-CSF, IFNγ, IL-1 β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-23 and TNFα.

Price: $24.69/ sample (single analysis)

Volume Required: 50µl single analysis. 75µl duplicate analysis. 100µl triplicate analysis.

Biomarker Research Areas: Immune response, cardiovascular disease, diabetes, neurological disorders and cancer.

Multiplex Assay Overview: The assay service provided by Eve Technologies on their Human High Sensitivity T-Cell Discovery Array 14-Plex facilitates research on immune response, cardiovascular disease, diabetes, neurological disorders and cancer. This Discovery Assay® measures fourteen biomarkers simultaneously in a single microwell. The assay is designed for extracellular protein analysis in nearly all types of biological samples.

Background Information: Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Intended Sample Type: Human serum or plasma (EDTA recommended), cell cultures, tissue homogenates and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation: ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane.

All samples will be diluted 2-fold by Eve Technologies, as recommended by Millipore for this assay.

Usage Statement: Unless otherwise stated our products and services are intended for research use only.

Detection Method: Multiplex Immunoassay analyzed with a BioPlex 200

Human High Sensitivity T-Cell Discovery Array 14-Plex Assay Kit Source: Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy

*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.

% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay Inter-assay Accuracy
pg/mL %CV %CV % Recovery
GM-CSF 0.35 < 5 < 15 101
IFNγ 0.48 < 5 < 20 101
IL-1β 0.14 < 5 < 15 98
IL-2 0.19 < 5 < 15 103
IL-4 1.12 < 5 < 15 103
IL-5 0.12 < 5 < 20 101
IL-6 0.11 < 5 < 20 107
IL-8 0.13 < 5 < 15 103
IL-10 0.56 < 5 < 20 104
IL-12 (p70) 0.15 < 6 < 15 100
IL-13 0.23 < 5 < 20 103
IL-17A 0.33 < 5 < 20 106
IL-23 3.25 < 5 < 20 100
TNFα 0.16 < 5 < 15 103
Normal Reference Range TBD

 

Multiplex Assay Methods

Below is a methods description that is often used in publications.

Using a methods description that credits Eve Technologies in a publication will result in a 10% discount on your next order!

Multiplex analysis of cytokines

In this study we quantified 14 cytokine/ chemokine/ growth factor biomarkers simultaneously by using a Discovery Assay® called the Human High Sensitivity T-Cell Discovery Array 14-Plex (Eve Technologies Corp, Calgary, AB, Canada).  The multiplex assay was performed at Eve Technologies by using the Bio-Plex™ 200 system (Bio-Rad Laboratories, Inc., Hercules, CA, USA), and a Milliplex Human High Sensitivity T-Cell panel (Millipore, St. Charles, MO, USA) according to their protocol.  The 14-plex consisted of GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-23, and TNFα. The assay sensitivities of these markers range from 0.11 – 3.25 pg/mL.  Individual analyte values and other assay details are available on Eve Technologies’ website or in the Milliplex protocol.

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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