Human Serum Amyloid A & ADAMTS13 Discovery Assay® Array (HDSAA)

We will test your sample(s) and provide results for the following biomarkers:

SAA, ADAMTS13

Discovery Price $16.99/sample (single analysis – includes sample filtration)

Single analysis (Tissue Homogenate/Cell Culture/Other)
Minimum volume required: 50 uL
Preferred volume: 75 uL

Duplicate analysis (Tissue Homogenate/Cell Culture/Other) 
Minimum volume required: 75 uL
Preferred volume: 100 uL

Single/duplicate analysis (Serum/Plasma)
Minimum volume required: 35 uL

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid

recommend Hyaluronic treatment for better assay performance. $3.99/sample

Tissue Homogenate
Tissue/Cell Culture Supernatant
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel) $3.00 per tube

No
Yes

(Category A)

Yes

(Category B)

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SKU: HDSAA Category: Tag:

Description

Biomarker Analytes:

SAA, ADAMTS13

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Human Serum Amyloid A & ADAMTS13 Array measures two cytokine receptor biomarkers simultaneously in a single microwell: SAA and ADAMTS13. The assay is designed for protein analysis in serum, plasma, cell culture, and tissue homogenate.

Intended Sample Type:

Human serum, plasma (EDTA recommended), cell cultures, and tissue homogenates. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane.

Serum and Plasma samples will be diluted 1:100 by Eve Technologies to bring protein concentrations within the standard curve range, as per the manufacturers instructions for use.

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Luminex xMAP technology is used for multiplexed quantification of 2 Human cytokines, chemokines, and growth factors.

The multiplexing analysis is performed using the Luminex™ 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta). Two markers are simultaneously measured in the samples using Eve Technologies’ Human SAA & ADAMTS13 2-Plex Discovery Assay® (MilliporeSigma, Burlington, Massachusetts, USA) according to the manufacturer’s protocol.  The 2-plex consists of SAA and ADAMTS13.  Assay sensitivities of these markers range from 0.093 – 0.339 ng/mL for the 2-plex.  Individual analyte sensitivity values are available in the MilliporeSigma MILLIPLEX® MAPprotocol or in the table below.

Cytokine Array Assay Kit Source:

Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy

*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.

% Recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay Inter-assay Accuracy
Analyte ng/mL %CV %CV % Recovery
SAA 0.093 <15 <20 96
ADAMTS13 0.339 <15 <20 94

Reference Ranges

25th percentile to 75th percentile (50% of samples tested have been observed to be within this range. 25% have been observed to be below this range and 25% have been observed to be above this range).

*For interpretation of clinical diagnostic (RUO) reports only*

Analyte Plasma (ng/mL)
Percentile  25th 75th
SAA 3271 18662
ADAMTS13 511 1076

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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