Multiplex Assay Overview:
The assay service provided by Eve Technologies on their Human Serum Amyloid A & ADAMTS13 Array measures two cytokine receptor biomarkers simultaneously in a single microwell: SAA and ADAMTS13. The assay is designed for protein analysis in serum, plasma, cell culture, and tissue homogenate.
Intended Sample Type:
Human serum, plasma (EDTA recommended), cell cultures, and tissue homogenates. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.
Special Sample Preparation:
ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane.
Serum and Plasma samples will be diluted 1:100 by Eve Technologies to bring protein concentrations within the standard curve range, as per the manufacturers instructions for use.
Unless otherwise stated our products and services are intended for research use only.
Luminex xMAP technology is used for multiplexed quantification of 2 Human cytokines, chemokines, and growth factors.
The multiplexing analysis is performed using the Luminex™ 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta). Two markers are simultaneously measured in the samples using Eve Technologies’ Human SAA & ADAMTS13 2-Plex Discovery Assay® (MilliporeSigma, Burlington, Massachusetts, USA) according to the manufacturer’s protocol. The 2-plex consists of SAA and ADAMTS13. Assay sensitivities of these markers range from 0.093 – 0.339 ng/mL for the 2-plex. Individual analyte sensitivity values are available in the MilliporeSigma MILLIPLEX® MAPprotocol or in the table below.
Cytokine Array Assay Kit Source:
Sensitivity, Variation, & Accuracy
*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% Recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.
25th percentile to 75th percentile (50% of samples tested have been observed to be within this range. 25% have been observed to be below this range and 25% have been observed to be above this range).
*For interpretation of clinical diagnostic (RUO) reports only*