Human Soluble Cytokine Receptor Array 14-plex (HDSCR14)

We Will Test Your Sample(s) and Provide Results For The Following Biomarkers:

sCD30, sEGFR, sgp130, sIL-1RI, sIL-1RII, sIL-2Ra, sIL-4R, sIL-6R, sRAGE, sTNF RI, sTNF RII, sVEGF R1, sVEGF R2, sVEGF R3

Discovery Price $23.99/sample (single analysis / includes sample filtration)
Single analysis volume required: *see note
Duplicate analysis volume required: *see note
Recommended Dilution: 
*Note: Single analysis: 25uL serum/plasma; 50uL cell culture. Duplicate analysis: 35uL serum/plasma; 75uL cell culture.

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel)

No
Yes

(Category A)

Yes

(Category B)

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SKU: HDSCR14 Category: Tag:

Description

Biomarker Analytes:

sCD30, sEGFR, sgp130, sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNF RI, sTNF RII, sVEGF R1, sVEGF R2, sVEGF R3.

Price:

$23.99 / sample (single analysis)

Volume Required:

Serum & Plasma: 30μL for single, 40μL duplicate, 50μL triplicate analysis.

Other sample types: 50μL single analysis. 75μL duplicate analysis. 100μL triplicate analysis.

Biomarker Research Areas:

Inflammatory response, various diseases.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Human Soluble Cytokine Receptor Array facilitates advanced inflammatory response research for almost all disease states. This Discovery Assay® measures 14 cytokine receptor biomarkers simultaneously in a single microwell: sCD30, sEGFR, sgp130, sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNF RI, sTNF RII, sVEGF R1, sVEGF R2, sVEGF R3. The assay is designed for protein analysis in serum, plasma, cell culture, and tissue homogenate. Other sample types may also be tested

Background Information:

Cytokines play a fundamental role in the pathogenesis of many diseases and are key targets for drug development. Like cytokines, soluble cytokine receptors are involved in regulating inflammatory responses and modulating immune events. This has stimulated significant research interest in their potential role as biomarkers for various diseases and immunotherapeutic agents. Soluble cytokine receptors naturally arise from genes encoding membrane- bound receptors or are direct derivatives of the receptors themselves. Many of these soluble cytokine receptors have the ability to inhibit the binding and biological activity of their cytokine ligands, making them very specific cytokine antagonists.

The assay service provided on this array is designed for the simultaneous analysis of multiple cytokine receptor biomarkers, supplying you with quantified results for the study of inflammatory and immune responses in serum, plasma, cell culture and tissue homogenate.

Intended Sample Type:

Human serum or plasma (EDTA recommended), cell culture, and tissue homogenate. A pilot run is recommended for cell culture, tissue homogenate and other sample types not listed.

Special Sample Preparation:

Serum and Plasma samples will be diluted 1:5 by Eve Tech to bring protein concentrations within the standard curve range.

Usage Statement:

For research use only.

Detection Method:

Multiplex Immunoassay analyzed with a BioPlex 200

Soluble Cytokine Receptor Assay Kit Source:

Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay Inter-assay Accuracy
pg/mL %CV %CV % Recovery
sCD30 7 <10 <15 101
sEGFR 42 <10 <15 101
sgp130 6 <10 <15 103
sIL-1RI 21 <10 <15 103
sIL-1RII 115 <10 <15 96
sIL-2Rα 11 <10 <15 91
sIL-4R 14 <10 <15 104
sIL-6R 9 <10 <15 103
sRAGE 3 <10 <15 104
sTNFRI 12 <10 <15 67
sTNFRII 8 <10 <15 99
sVEGFR1 111 <10 <15 98
sVEGFR2 71 <10 <15 101
sVEGFR3 47 <10 <15 96

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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