sCD30, sEGFR, sgp130, sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNF RI, sTNF RII, sVEGF R1, sVEGF R2, sVEGF R3.
$23.99 / sample (single analysis)
Serum & Plasma: 30μL for single, 40μL duplicate, 50μL triplicate analysis.
Other sample types: 50μL single analysis. 75μL duplicate analysis. 100μL triplicate analysis.
Biomarker Research Areas:
Inflammatory response, various diseases.
Multiplex Assay Overview:
The assay service provided by Eve Technologies on their Human Soluble Cytokine Receptor Array facilitates advanced inflammatory response research for almost all disease states. This Discovery Assay® measures 14 cytokine receptor biomarkers simultaneously in a single microwell: sCD30, sEGFR, sgp130, sIL-1RI, sIL-1RII, sIL-2Rα, sIL-4R, sIL-6R, sRAGE, sTNF RI, sTNF RII, sVEGF R1, sVEGF R2, sVEGF R3. The assay is designed for protein analysis in serum, plasma, cell culture, and tissue homogenate. Other sample types may also be tested
Cytokines play a fundamental role in the pathogenesis of many diseases and are key targets for drug development. Like cytokines, soluble cytokine receptors are involved in regulating inflammatory responses and modulating immune events. This has stimulated significant research interest in their potential role as biomarkers for various diseases and immunotherapeutic agents. Soluble cytokine receptors naturally arise from genes encoding membrane- bound receptors or are direct derivatives of the receptors themselves. Many of these soluble cytokine receptors have the ability to inhibit the binding and biological activity of their cytokine ligands, making them very specific cytokine antagonists.
The assay service provided on this array is designed for the simultaneous analysis of multiple cytokine receptor biomarkers, supplying you with quantified results for the study of inflammatory and immune responses in serum, plasma, cell culture and tissue homogenate.
Intended Sample Type:
Human serum or plasma (EDTA recommended), cell culture, and tissue homogenate. A pilot run is recommended for cell culture, tissue homogenate and other sample types not listed.
Special Sample Preparation:
Serum and Plasma samples will be diluted 1:5 by Eve Tech to bring protein concentrations within the standard curve range.
For research use only.
Multiplex Immunoassay analyzed with a BioPlex 200
Soluble Cytokine Receptor Assay Kit Source:
Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.