Mouse Cytokine/Chemokine 31-Plex Discovery Assay® Array (MD31)

Rated 5.00 out of 5 based on 3 customer ratings
(3 customer reviews)

We will test your sample(s) and provide results for the following biomarkers:

Eotaxin, G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα, VEGF-A 

Discovery Price $41.99/sample (single analysis – includes sample filtration)

Single analysis
Minimum volume required: 50 uL
Preferred volume: 75 uL

Duplicate analysis
Minimum volume required: 75 uL
Preferred volume: 100 uL

Recommended Dilution: Serum and Plasma are recommended to be run at a 2-fold dilution. If you wish to run your samples at a dilution, predilute your samples with PBS pH~7.5. For example, add 25ul of serum to 25ul of PBS for single analysis volume of 50ul. Indicate your dilution factor on the order form.          

If you expect your samples to have low cytokine levels, i.e. normal healthy populations, you may wish to inquire about a High Sensitivity panel.        

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid

recommend Hyaluronic treatment for better assay performance. $3.99/sample

Tissue Homogenate
Tissue/Cell Culture Supernatant
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel) $3.00 per tube

No
Yes

(Category A)

Yes

(Category B)

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SKU: MD31 Category: Tag:

Description

Biomarker Analytes: Eotaxin, G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα, and VEGF

Volume Required:

50μl single analysis. 75μl duplicate analysis. 100μl triplicate analysis.
Biomarker Research Areas: Immune response, inflammatory disease, allergy, irritable bowel disease (IBD), sepsis and cancer.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Mouse Cytokine Array / Chemokine Array 32-Plex facilitates research on immune response, inflammatory disease, allergy, irritable bowel disease (IBD), sepsis and cancer. This Discovery Assay® measures thirty-two biomarkers simultaneously in a single microwell. The assay is designed for extracellular protein analysis in nearly all types of biological samples.

Background Information:

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Responses to cytokines result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity.

Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
Intended Sample Type: Mouse serum or plasma (EDTA recommended), cell cultures, tissue homogenates and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

Serum and Plasma are recommended to be run at a 2-fold dilution. If you wish to run your samples at a dilution, predilute your samples with PBS
pH~7.5.
ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Multiplex Immunoassay analyzed with a BioPlex 200 Mouse Cytokine Array/ Chemokine Array 32-Plex

Assay Kit Source:

Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy

Sensitivity Intra-assay Inter-assay Accuracy
Biomarkers pg/mL %CV %CV % Recovery
Eotaxin 0.4 2.1 4.4 98.1
G-CSF 0.5 2.6 5.1 91.6
GM-CSF 10.9 2.0 6.4 91.8
IFNγ 0.4 1.9 4.9 95.3
IL-1α 10.3 4.4 4.7 92.1
IL-1β 5.4 2.5 5.8 99.3
IL-2 0.6 1.5 3.8 91.2
IL-3 0.7 1.7 7.2 96.6
IL-4 0.2 1.7 5.1 95.0
IL-5 0.3 2.5 6.3 90.4
IL-6 0.4 2.3 5.4 92.2
IL-7 1.4 2.3 4.6 98.9
IL-9 17.3 2.7 4.9 101.6
IL-10 1.2 1.4 5.1 91.0
IL-12 (p40) 3.9 1.6 6.6 89.7
IL-12 (p70) 4.8 2.6 4.1 84.9
IL-13 33.3 2.5 ≤15 106.5
IL-15 7.4 2.2 7.2 89.5
IL-17 0.2 2.0 5.8 96.7
IP-10 0.1 2.2 3.4 94.8
KC 1.8 3.3 6.0 93.3
LIF 0.5 3.3 2.9 97.2
LIX 22.1 2.5 8.3 93.1
MCP-1 6.7 1.9 4.3 95.0
M-CSF 3.5 2.7 5.7 89.5
MIG 2.4 3.2 4.7 92.5
MIP-1α 7.7 1.5 5.5 96.7
MIP-1β 11.9 1.9 4.6 96.4
MIP-2 30.6 2.9 6.8 90.5
RANTES 2.7 4.9 12.4 95.0
TNFα 1.7 2.6 9.3 94.0
VEGF 0.1 2.4 3.9 101.0

*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.

% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

3 reviews for Mouse Cytokine/Chemokine 31-Plex Discovery Assay® Array (MD31)

  1. Rated 5 out of 5

    (verified owner)

    did panel in mouse macrophages and happy with results!

  2. Rated 5 out of 5

    did a panel in mouse BALF, received great service throughout the process!

  3. Rated 5 out of 5

    (verified owner)

    Did this panel in mouse tumor-infiltrating lymphocytes from multiple models. Good data and fast service. Staffs are extremely helpful. Highly recommended!

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Customer
michael.melkus
1 year 9 months ago

Can HMGB1 be added to this assay?

 
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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