Mouse, Rat Metabolic Array (MRDMET)

We Will Test Your Sample(s) and Provide Results For The Following Biomarkers:

Amylin (active)*, C-Peptide 2, GIP(total), GLP-1(active)*, Ghrelin(active)*, Glucagon*, Insulin, Leptin, PP, PYY, Resistin (Mouse only)

Discovery Price $22.19/sample (single analysis / includes sample filtration)
Single analysis volume required: 35uL
Duplicate analysis volume required: 45uL
Recommended Dilution: 
*Note: Serum/plasma samples: at time of collection add DPPIV inhibitor (for GLP-1 measurement), Protease Inhibitor cocktail (for Amylin measurement), Aprotinin (for Glucagon measurement) and Serine protease inhibitor (for active ghrelin measurement).

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel)

No
Yes

(Category A)

Yes

(Category B)

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SKU: MRDMET Category: Tags: ,

Description

Biomarker Analytes:

Amylin (active), C-Peptide 2, GIP, GLP-1, Ghrelin, Glucagon, Insulin, Leptin, PP, PYY, Resistin.

Price:

$22.19 / sample (single analysis)

Volume Required:

35μl single analysis. 45μl duplicate analysis. 55μl triplicate analysis.

Biomarker Research Areas:

Metabolic disorders, diabetes, obesity, cardiovascular disease.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Mouse Metabolic 11-Plex facilitates research on Metabolic disorders, diabetes, obesity, and cardiovascular disease. This Discovery Assay® measures eleven metabolic biomarkers simultaneously in a single microwell: Amylin (active), C-Peptide 2, GIP, GLP-1, Ghrelin, Glucagon, Insulin, Leptin, PP, PYY, Resistin. The assay is designed for protein analysis in The assay is designed for protein analysis in serum, plasma, cell culture and tissue homogenates. Other samples can be tested, however we recommend a pilot run.

Background Information:

Metabolic syndrome is a cluster of conditions that occur together, including increased blood pressure, elevated insulin levels, excess body fat around the waist, and abnormal cholesterol levels. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity—all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death. Adults with metabolic syndrome present a low-grade inflammation, whose link with obesity may be a production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes and obesity related hormones, as well as other cardiovascular disease biomarkers.

Intended Sample Type:

Mouse serum or plasma (EDTA recommended), cell cultures, tissue homogenates and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

For serum and plasma samples, the measurement of certain targets requires the addition of inhibitors at the time of collection. They are as follows:

GLP-1 measurement : DPPIV inhibitor

Active Amylin measurement: Protease inhibitor

Glucagon measurement: Aprotinin

Active Ghrelin measurement: Serine protease inhibitor

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Multiplex Immunoassay analyzed with a BioPlex 200

Mouse Metabolic 11-Plex Array Assay Kit Source:

Millipore MILLIPLEX

 

Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay Inter-assay Accuracy
pg/mL %CV %CV % Recovery
Amylin (active) 19 7 10 104
C-Peptide 2 20 4 8 89
GIP 1 7 7 97
GLP-1 23 5 12 102
Ghrelin 3 5 7 92
Glucagon 7 13 10 115
Insulin 14 5 7 99
Leptin 19 5 7 93
PP 2 7 9 92
PYY 5 8 11 84
Resistin 23 6 17 96

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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