Porcine (Pig) Cytokine Array / Chemokine Array 13-plex (PD13)

We Will Test Your Sample(s) and Provide Results For The Following Biomarkers: GM-CSF, IFNgamma, IL-1alpha, IL-1ra, IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, TNF-alpha

Discovery Price $24.99/sample (single analysis / includes sample filtration)

Single analysis volume required: 50uL Duplicate analysis volume required: 75uL

Please Click for information on Shipping, Tubes, Volumes, Dilution and Labeling for Discovery Assays

Human
Mouse
Rat
Porcine
Non Human Primate
Canine
Feline
Horse
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Additional Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No Tertiary Sample Type
BAL Fluid
Cell Lysate
CSF
Plasma-Citrate
Plasma-EDTA
Plasma-Heparin
Serum
Synovial Fluid
Tissue Homogenate
Tissue/Cell Culture
Urine
Other
No
Yes, 2 Fold

(1 Part Sample and 1 Part Diluent)

Yes, Other

Please Specify in the "Special Requests" Notes at Checkout

0.5-0.65mL Snap Cap

(Discovery Assay Compatible)

1.5mL Snap Cap

(Non Compatible Tubes $3.00/ Tube)

96-Well Plate

(Non Compatible Tubes $3.00/ Tube)

Cryo Screw Top Tube

(Non Compatible Tubes $3.00/ Tube)

Other

(Non Compatible Tubes $3.00/ Tube)

Alpha-Numerically

(ie. S1,S2,S3)

Other

Please send Sample list (excel)

No
Yes

(Category A)

Yes

(Category B)

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SKU: PD13 Category: Tag:

Description

Biomarker Analytes:

GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, TNFα

Price:

$24.99 / sample (single analysis)

Volume Required:

50μL single analysis. 75μL duplicate analysis. 100μL triplicate analysis.

Biomarker Research Areas:

Inflammatory disease, immune response, cardiovascular disease, cancer, general herd health, and porcine animal models.

Multiplex Assay Overview:

The assay service provided by Eve Technologies on their Pig Cytokine Array facilitates research in a numerous research areas and acts as an important tool to monitor pig and corresponding herd health. This Discovery Assay® measures 13 cytokine / chemokine biomarkers simultaneously in a single microwell: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, TNFα. The assay is designed for protein analysis in serum, plasma, cell culture, and tissue homogenate. Other sample types may also be tested.

Background Information:

The ability to analyze the expression of multiple cytokines and chemokines simultaneously in both farm and research model pigs is significant in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens. The inflammatory response is important in both normal conditions and numerous diseases such as inflammatory disease, cardiovascular disease, and cancer.

A close homology to humans have given pig models an advantage over other animal models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone research. The human similarities include similar heart size, blood flow rate, skin, liver enzymes and bone healing. “Mini pigs” in particular have hearts similar to humans with respect to size and function. Mini pigs also age without growing too large and they can be used for multi-generational studies that can be performed in a comparatively short period of time. long term preclinical studies can utilize this feature.

Intended Sample Type:

Pig serum or plasma (EDTA recommended), cell culture, and tissue homogenate. A pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.

Special Sample Preparation:

Usage Statement:

Unless otherwise stated our products and services are intended for research use only.

Detection Method:

Multiplex Immunoassay analyzed with a BioPlex 200

Cytokine Array Assay Kit Source:

Millipore MILLIPLEX

Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.

Sensitivity Intra-assay Inter-assay Accuracy
pg/mL %CV %CV % Recovery
GM-CSF 20.0 <10 <20 103
IFNg 42.0 <10 <20 94
IL-1 alpha 5.0 <10 <20 99
IL-1 beta 42.0 <10 <20 100
IL-1ra 10.0 <10 <20 90
IL-2 8.0 <10 <20 95
IL-4 20.0 <10 <20 95
IL-6 9.0 <10 <20 92
IL-8 5.0 <10 <20 96
IL-10 9.0 <10 <20 99
IL-12 5.0 <10 <20 100
IL-18 12.0 <10 <20 101
TNFa 6.0 <10 <20 99

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Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
Resources
Please See our Documentation related to Sample Dilution, Shipping of Biological Samples, and other related information.
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