GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, TNFα
$24.99 / sample (single analysis)
50μL single analysis. 75μL duplicate analysis. 100μL triplicate analysis.
Biomarker Research Areas:
Inflammatory disease, immune response, cardiovascular disease, cancer, general herd health, and porcine animal models.
Multiplex Assay Overview:
The assay service provided by Eve Technologies on their Pig Cytokine Array facilitates research in a numerous research areas and acts as an important tool to monitor pig and corresponding herd health. This Discovery Assay® measures 13 cytokine / chemokine biomarkers simultaneously in a single microwell: GM-CSF, IFNγ, IL-1α, IL-1ra, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18, TNFα. The assay is designed for protein analysis in serum, plasma, cell culture, and tissue homogenate. Other sample types may also be tested.
The ability to analyze the expression of multiple cytokines and chemokines simultaneously in both farm and research model pigs is significant in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens. The inflammatory response is important in both normal conditions and numerous diseases such as inflammatory disease, cardiovascular disease, and cancer.
A close homology to humans have given pig models an advantage over other animal models in many research areas such as cardiovascular, toxicology, pharmacology, aging and bone research. The human similarities include similar heart size, blood flow rate, skin, liver enzymes and bone healing. “Mini pigs” in particular have hearts similar to humans with respect to size and function. Mini pigs also age without growing too large and they can be used for multi-generational studies that can be performed in a comparatively short period of time. long term preclinical studies can utilize this feature.
Intended Sample Type:
Pig serum or plasma (EDTA recommended), cell culture, and tissue homogenate. A pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.
Special Sample Preparation:
Unless otherwise stated our products and services are intended for research use only.
Multiplex Immunoassay analyzed with a BioPlex 200
Cytokine Array Assay Kit Source:
Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.