EGF, Eotaxin, Fractalkine, G-CSF, GM-CSF, GRO/KC/CINC-1, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IL-18, IP-10, Leptin, LIX, MCP-1, MIP-1α, MIP-2, RANTES, TNFα and VEGF
50µl single analysis. 75µl duplicate analysis. 100µl triplicate analysis.
Biomarker Research Areas:
Immune response, inflammation, allergy, irritable bowel disease (IBD), sepsis, and cancer.
Multiplex Assay Overview:
The assay service provided by Eve Technologies on their Rat Cytokine Array/ Chemokine Array 27-Plex facilitates research in any area that involves the immune system such as inflammation, allergies, irritable bowel disease (IBD), sepsis, and cancer. This Discovery Assay® measures twenty-seven biomarkers simultaneously in a single microwell. The assay is designed for extracellular protein analysis in nearly all types of biological samples. Background Information: “Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
Intended Sample Type:
Rat serum, plasma (EDTA recommended), cell cultures, tissue homogenates, synovial fluid, CSF, sputum, BAL, urine and others. Due to a wide range of sample conditions, a pilot run is recommended for cell cultures, tissue homogenates, and other sample types not listed.
Special Sample Preparation:
ALL samples must be centrifuged prior to aliquoting. The aliquot should be drawn from the middle of the sample volume. Samples that have a high amount of contaminants, cellular debris, lipids, or coagulation can result in a volume loss as volume is being displaced by this debris and they can clog the filters. Highly turbid or coagulated samples that clog the filters will be partially filtered by piercing the clogged membrane. Serum and Plasma are recommended to be run at a 2-fold dilution. If you wish to run your samples at a dilution, predilute your samples with PBS pH~7.5. For example, add 25ul of serum to 25ul of PBS for single analysis volume of 50ul. Indicate your dilution factor on the order form.
Unless otherwise stated our products and services are intended for research use only.
Multiplex Immunoassay analyzed with a BioPlex 200 Cytokine Array Assay Kit Source: Millipore MILLIPLEX