TGF-β1, TGF-β2, TGF-β3
Biomarker Research Areas:
Immune response, angiogenesis, wound healing, embryonic development, heart disease, cancer.
Multiplex Assay Overview:
The assay service provided by Eve Technologies on their TGF-beta 3-Plex facilitates research in various fields including Immune response, angiogenesis, wound healing, embryonic development, heart disease, and cancer. This Discovery Assay® measures 3 TGFβ biomarkers simultaneously in a single microwell: TGF-β1, TGF-β2, TGF-β3. The assay is designed for protein analysis in a variety of sample types and in multiple species. Due to samples requiring an acid-treatment step, the TGFβ biomarkers are assayed separately from other cytokines.
The transforming growth factor beta (TGF-β) system, a superfamily of cytokines as well as signalling pathways, is highly conserved throughout the animal kingdom. TGF-β functions in angiogenesis, wound healing and embryonic development, and plays a critical role in immunity, heart disease, and cancer. In its normal state TGF-β is one of the few classes of
proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGF-β signalling pathway result in tumor cell resistance to the effects of normally functioning TGF-β, causing proliferation without regulation. Initial research suggests that VE-cahedrin may enhance the mutated TGF-β signaling pathway, while other research indicates that DNA methylation plays a role in pathway mutation.
The secreted TGF-β cytokine exists in three isoforms: TGF-β1, TGF-β2 and TGF-β3. Secreted by most immune cells, TGF-β1 plays a critical role in controlling the immune system, acting on cells differently depending on cell type as well as stage of differentiation. TGF-β2, also known as glioblastoma-derived T-cell suppressor factor (G-TSF), plays a role in embryonic development and has the ability to suppress the effects of interleukin-dependent T-cell tumors. TGF-β3 regulates cellular adhesion
molecules and extracellular matrix formation, as well as lung and palate development. TGF-β3 deficiency during mammalian development results in the cleft palate deformity. In addition TGF-β3 controls wound healing by regulating epidermal and dermal cell movement in injured skin.
Note: TGF-β3 may not be detected in normal plasma and serum samples.
Intended Sample Type:
Since TGF-β is highly conserved throughout the animal kingdom, this is a multi-species assay that can be tested with various sample types. Specific species include: Human, mouse, rat, pig, horse, rabbit, and guinea pig.
Due to the involvement of all three isoforms in neonate development and lactation regulation, literature suggests detectable levels in milk.
Special Sample Preparation:
Samples that are designated for TGF-β analysis are treated by Eve Technologies with hydrochloric acid – a necessary step to disassociate TGF-β1 from Latency Associated Peptide (LAP).
It is also possible that some serum and plasma samples may contain low
levels of immunoreactive TGF beta 1 that has disassociated from LAP. Naturally occurring, free TGF beta 1 may be measurable in this assay by evaluating samples without acid treatment. If you wish to detect natural free TGF-β1 please write “Do NOT acid treat samples” in the Additional Comments section of your order form.
Serum and Plasma samples will be diluted 1:30 by Eve Tech to bring protein concentrations within the standard curve range.
Unless otherwise stated our products and services are intended for research use only.
Multiplex Immunoassay analyzed with a BioPlex 200
Cytokine Array Assay Kit Source:
Sensitivity, Variation, & Accuracy*Note: %CV is an appropriate measure of quality only for sample that have significant detectable concentrations for a particular analyte. Low concentration / low signal samples can often have a much higher %CV as the signals are at the limitations of the assay and fall within a flat portion of the curve.
% recovery is comparing the observed concentration to the expected concentration of samples spiked with the standard curve cocktail at various concentrations.