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Human MMP and TIMP 13-Plex Discovery Assay® Array for Cell Culture and non-blood samples (HMMP/TIMP-C,O)

$31.99

Biomarkers
MMP-1 (Collagenase 1) | MMP-2 (Gelatinase A)* | MMP-3 (Stromelysin 1) | MMP-7 (Matrilysin) | MMP-8 (Collagenase 2) | MMP-9 (Gelatinase B)** | MMP-10 (Matrix Metalloproteinase 10) | MMP-12 (Macrophage Metalloelastase) | MMP-13 (Collagenase 3)*** | TIMP-1 | TIMP-2 | TIMP-3**** | TIMP-4

****The kit manufacturer has removed TIMP-3 from this panel. Whilst we have this target available at the moment, wells are limited. 

Price
$31.99/sample (single analysis)

Sample Volume Requirements
50 μL 

*Cell culture supernate samples are not suitable for MMP-2.
**To measure circulating MMP-9 in plasma it is necessary to remove platelets from the samples.
***To measure circulating MMP-13, plasma and platelet-poor plasma samples are not suitable.
EDTA and Citrate are not recommended for use in this assay due to their chelating properties.
Hemolyzed and icteric samples are not suitable for use in this assay.
See our Sample Preparation Guide for more information

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type (Fee applies)

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout, fee applies)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions.) We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect. We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

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Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Consider this assay for a comprehensive analysis of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in cell culture and tissue homogenate and non-blood biological samples such as urine, CSF and saliva.

This advanced array features two assays conducted simultaneously from a single small sample volume. By running both assays concurrently, we deliver efficient, high-quality results, providing insights from two distinct assays with a rapid turnaround time.

The Human MMP and TIMP 13-Plex Discovery Assay® Array (HMMP/TIMP-C,O) measures MMP-1, MMP-2, MMP-9, TIMP-1, and TIMP-4, among others, providing insights into extracellular matrix remodeling and tissue homeostasis.

TIMPs play a crucial role in regulating MMP activity, and an imbalance between these molecules is linked to conditions like arthritis, cardiovascular disorders, and cancer. For example, MMP-9 is a key marker in cancer metastasis and chronic inflammation. TIMP levels can serve as valuable cancer biomarkers, reflecting tumor progression and survival. Additionally, TIMPs have unique functions beyond MMP inhibition, such as TIMP-1’s role in enhancing erythroid activity and in inhibiting excessive matrix degradation and TIMP-2’s suppression of EGF-mediated signaling.

Ideal for studies focused on cancer progression, tissue fibrosis, and inflammatory diseases, this assay helps elucidate the role of MMPs and TIMPs in pathological processes.

Please note that the manufacturer has removed TIMP-3 from this panel. While we still have TIMP-3 available, the wells are limited, so availability may vary.

Click here for information on shipping biological samples for Discovery Assays