Sample Dilution Guide

Sample Dilution Guide

Samples are run as received and as per the kit manufacturer’s instructions for use, with the exception of a few Mouse and Rat Discovery Assays.

The manufacturer of some of the rodent kits recommends serum/plasma to be run at a 2-fold dilution. This is indicated partly for the purpose of breaking up the stronger matrix that is in rodent blood and may result in increasing signal for some analytes by ameliorating issues with the serum matrix. Some clients however wish for their samples to be run undiluted with the perception that this might increase sensitivity. We therefore run samples as received (in the format supplied to us) for the following Discovery Assays: MD44, MD32, MD13, MDF10, RD27. If you would like your serum/plasma samples run at the kit protocol recommended 2-fold dilution for these assays, you may dilute your samples prior to shipping by following the guidelines below, or you can request Eve Technologies dilute your samples prior to assay performance for a fee of $4 per sample.

All other assays are run as per the manufacturer’s recommended dilutions.

If you have purchased a Custom Plex kit which requires dilution of samples, Eve Technologies will reach out to you prior to assay performance to discuss a pilot analysis of a select few samples at a range of dilutions to determine the optimal dilution factor for your samples (this is part of the Custom Plex assay service and is included in the overall cost).

How to dilute your samples:

Dilute your samples in standard PBS pH ~7.5.
2-Fold Dilution:  1 part sample to 1 part buffer (25ul sample to 25ul buffer)
3-Fold Dilution:  1 part sample to 2 parts buffer (25ul sample to 50ul buffer)
4-Fold Dilution: 1 part sample to 3 parts buffer (25ul sample to 75ul buffer), etc.

Note: The results of samples run at different dilutions cannot be compared against each other directly as the effect of a dilution is not linear due to a sigmoidal standard curve and matrix effects associated with dilution. Dilutional linearity cannot be assumed and if you intend to compare all samples together in your analysis, all samples should be run under the same conditions (i.e., same dilution) and treated in the same manner for each individual assay.

Reminder: Please aliquot and label samples in accordance with our Conforming Tubes and Sample Labeling Instructions.  Proper labeling and documentation of samples are essential for accurate tracking and interpretation of results.