Sample Dilution Guide

Sample Dilution Guide

Samples are run as received and according to the kit manufacturer’s instructions for use. If the kit manufacturer requires samples to be diluted, Eve Technologies will perform the dilution as part of the assay service.

If you have purchased a Custom Plex kit that requires sample dilution, Eve Technologies will contact you before assay performance to discuss a pilot analysis. This pilot analysis involves running a select number of samples at a range of dilutions to determine the optimal dilution factor for your samples. This is part of the Custom Plex assay service and is included in the overall cost.

Note: Results from samples run at different dilutions cannot be directly compared. The effect of dilution is not linear due to the sigmoidal standard curve and matrix effects associated with dilution. Dilutional linearity cannot be assumed.

If you intend to compare all samples together in your analysis, all samples should be run under the same conditions, including the same dilution, and treated in the same manner for each individual assay.

Reminder: Please aliquot and label samples in accordance with our Conforming Tubes and Sample Labeling Instructions.  Proper labeling and documentation of samples are essential for accurate tracking and interpretation of results.