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Mouse Cytokine Proinflammatory Focused 10-Plex Discovery Assay® Array (MDF10)

$28.99

Biomarkers
GM-CSF | IFNγ | IL-1β | IL-2 | IL-4 | IL-6 | IL-10 | IL-12p70 | MCP-1 | TNFα

Price
$28.99/sample (single analysis)

Sample Volume Requirements
Single analysis 75 μL
Duplicate analysis 100 μL

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Species Type

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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type (Fee applies)

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout, fee applies)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions.) We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect. We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

Tube Type:

Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Consider this assay for a focused analysis of key proinflammatory cytokines in mouse models. The Mouse Cytokine Proinflammatory Focused 10-Plex Discovery Assay® Array (MDF10) measures GM-CSF, IFNγ, IL-1β, IL-6, and TNFα, among others, providing insights into inflammation and immune response.

Ideal for studying autoimmune diseases such as rheumatoid arthritis, inflammatory conditions like sepsis, and infection responses, this assay helps elucidate the role of critical cytokines in disease progression. For instance, IL-6 and TNFα are key markers in chronic inflammation, while GM-CSF and IFNγ are crucial in immune regulation. If you anticipate that your samples may have low cytokine levels, such as those from normal healthy populations, you might want to consider our High Sensitivity panel. This advanced option is specifically designed to detect low abundance cytokines with greater precision, ensuring you capture even subtle variations in your data.

Discover the power of precision with our Mouse Cytokine Proinflammatory Focused 10-Plex Discovery Assay®, expertly designed for the Luminex® 200™ platform. This cutting-edge technology enables the simultaneous detection of multiple biomarkers from a minimal single sample, delivering comprehensive insights with unmatched efficiency. Streamline your research and unlock a new level of detail with our advanced multiplex assay solution.

Recommended Dilution: The kit manufacturer recommends Serum and Plasma be run at a 2-fold dilution. If you wish to run your samples at a dilution, predilute your samples with PBS pH~7.5. See our Sample Dilution Guide for more information.

If you expect your samples to have low cytokine levels, i.e. normal healthy populations, you may wish to inquire about a High Sensitivity panel. 

Click here for information on shipping biological samples for Discovery Assays    

Published Research Featuring Our MDF10:
  1. Zhu, X., Li, Q., George, V., Spanoudis, C., Gilkes, C., Shrestha, N., Liu, B., Kong, L., You, L., Echeverri, C., Li, L., Wang, Z., Chaturvedi, P., Muniz, G. J., Egan, J. O., Rhode, P. R., & Wong, H. C. (2023). A novel interleukin-2-based fusion molecule, HCW9302, differentially promotes regulatory T cell expansion to treat atherosclerosis in mice. Frontiers in Immunology, 14, 1114802.  
  2. Hamon, P., Gerbé De Thoré, M., Classe, M., Signolle, N., Liu, W., Bawa, O., Meziani, L., Clémenson, C., Milliat, F., Deutsch, E., & Mondini, M. (2022). TGFβ receptor inhibition unleashes interferon-β production by tumor-associated macrophages and enhances radiotherapy efficacy. Journal for ImmunoTherapy of Cancer, 10(3), e003519.
  3. Kim, J. E., Tung, L. T., Jiang, R. R., Yousefi, M., Liang, Y., Malo, D., Vidal, S. M., & Nijnik, A. (2023). Dysregulation of B lymphocyte development in the SKG mouse model of rheumatoid arthritis. Immunology, 170(4), 553–566.