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Human Cytokine/Chemokine 96-Plex Discovery Assay® Array (HD96)

$135.99

Biomarkers
6Ckine | APRIL | BAFF | BCA-1 | CCL28 | sCD137 | sCD40L | CTACK | CXCL16 | EGF | ENA-78 | Eotaxin | Eotaxin-2 | Eotaxin-3 | sFas | sFasL | FGF-2 | FLT-3L | Fractalkine | GCP-2 | G-CSF | GM-CSF | Granzyme A | Granzyme B | GROα | HMGB1 | I-309 | IFN-α2 | IFNβ | IFNꙍ | IFNγ | IL-1α | IL-1β | IL-1RA | IL-2 | IL-3 | IL-4 | IL-5 | IL-6 | IL-7 | IL-8 | IL-9 | IL-10 | IL-11 | IL-12p40 | IL-12p70 | IL-13 | IL-15 | IL-16 | IL-17A | IL-17E/IL-25 | IL-17F | IL-18 | IL-20 | IL-21 | IL-22 | IL-23 | IL-24 | IL-27 | IL-28A | IL-29 | IL-31 | IL-33 | IL-34 | IL-35 | IP-10 | I-TAC | LIF | Lymphotactin | MCP-1 | MCP-2 | MCP-3 | MCP-4 | M-CSF | MDC | MIG/CXCL9 | MIP-1α | MIP-1β | MIP-1Ᵹ | MIP-3α | MIP-3β | MPIF-1 | PDGF-AA | PDGF-AB/BB | Perforin | RANTES | SCF | SDF-1 | TARC | TGFα | TNFα | TNFβ | TPO | TRAIL | TSLP | VEGF-A

Price
$135.99/sample
(single analysis)

Sample Volume Requirements
Single analysis 100 μL
Duplicate analysis 150 μL

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Species Type

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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions)

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect)

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

Tube Type:

Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Explore the most comprehensive cytokine and chemokine profile available with the Human Cytokine/Chemokine 96-Plex Discovery Assay® Array (HD96)! This advanced array features two assays conducted simultaneously from a single small sample volume: HD48A and HD48B. By running both assays concurrently, we deliver efficient, high-quality results, providing insights from two distinct assays with a rapid turnaround time.

The HD96 assay offers an unparalleled, broad, and detailed view of the circulating immune microenvironment across a wide range of both acute and chronic inflammatory conditions, as well as autoimmune and autoinflammatory diseases. It excels in research on complex diseases such as cancer, autoimmune disorders, cardiovascular diseases, and chronic infections.

For example, biomarkers like IL-6 and TNFα are crucial for studying chronic inflammatory diseases such as rheumatoid arthritis and lupus, while VEGF-A and PDGF-AA are key to exploring tumor angiogenesis and progression. Additionally, the panel facilitates the characterization of type 1 (Th1), type 2 (Th2), and type 3 (Th17) immunity, enhancing your understanding of the immune response.

If you’re unsure of which biomarkers to focus on, the HD96 panel’s broad scope allows you to detect a wide range of targets and identify key biomarkers, which can then be refined in follow-up assays for more targeted analysis. This versatile and affordable option provides a broad array of insights, optimizing your workflow and accelerating your research outcomes.

Discover the power of precision with our assay, expertly designed for the Luminex® 200™ platform. This cutting-edge technology enables the simultaneous detection of multiple biomarkers from a minimal single sample, delivering comprehensive insights with unmatched efficiency. Streamline your research and unlock a new level of detail with our advanced multiplex assay solution.

Recommended Dilution: Most samples are run undiluted, however RANTES has high concentrations in serum and plasma. If this target is important to your study and your sample type is serum or plasma, it is recommended to run samples in a separate assay with a dilution factor applied or utilize our Human Supplemental Biomarker 10-Plex (HDHSB10)

If you expect your samples to have low cytokine levels, i.e. normal healthy populations, you may wish to inquire about a High Sensitivity panel.

Click here for information on shipping biological samples for Discovery Assays 

Published Research Featuring Our HD96:
  1. Smith, L., Gonzalez Santiago, E., Eke, C., Gu, W., Wang, W., Llivichuzhca-Loja, D., Kehoe, T., St Denis, K., Strine, M., Taylor, S., Tseng, G., & Konnikova, L. (2024). Human milk supports robust intestinal organoid growth, differentiation, and homeostatic cytokine production. Gastro Hep Advances. Advance online publication.
  2. Chui, M. K., Schneider, K., Miclau, K., LaHue, S. C., Furman, D., Leutwyler, H., & Newman, J. C. (2024). Associations of systemic inflammation and senescent cell biomarkers with clinical outcomes in older adults with schizophrenia. medRxiv.
  3. Krumholz, H. M., Sawano, M., Bhattacharjee, B., Caraballo, C., Khera, R., Li, S.-X., Herrin, J., Coppi, A., Holub, J., Henriquez, Y., Johnson, M. A., Goddard, T. B., Rocco, E., Hummel, A. C., Mouslmani, M. A. L., Putrino, D. F., Carr, K. D., Carvajal-Gonzalez, S., Charnas, L., De Jesus, M., & Iwasaki, A. (2024). The PAX LC trial: A decentralized, phase 2, randomized, double-blind study of nirmatrelvir/ritonavir compared with placebo/ritonavir for long COVID. The American Journal of Medicine

 

NOTE: Results will not directly correlate with the previous HD42 or HD65 assay, as a result of the antibody systems being optimized in this new panel. If you are in need of the previous HD42 or HD65 assay in order to correlate with past data, please contact us directly.