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Porcine (Pig) Cytokine/Chemokine 13-Plex Discovery Assay® Array (PD13)

$32.99

Biomarkers
GM-CSF | IFNγ | IL-1α | IL-1RA | IL-1β | IL-2 | IL-4 | IL-6 | IL-8 | IL-10 | IL-12 | IL-18 | TNFα

Price
$32.99/sample
(single analysis)

Sample Volume Requirements
Single analysis 75 μL
Duplicate analysis 100 μL

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Species Type

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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions)

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect)

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

Tube Type:

Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Consider this assay for a focused analysis of 13 crucial cytokines and chemokines in porcine samples. The Porcine (Pig) Cytokine/Chemokine 13-Plex Discovery Assay® Array (PD13) includes essential biomarkers such as IL-6, TNFα, IL-10, and IFNγ, covering key aspects of immune and inflammatory responses in pigs.

This panel is valuable for research in veterinary immunology, infectious diseases, and inflammatory conditions in swine. For example, IL-6 and TNFα are critical in studying inflammatory responses and immune activation, while IL-10 provides insights into anti-inflammatory mechanisms.

The porcine model is also highly relevant for cardiovascular research due to its anatomical and physiological similarities to the human heart, significantly increasing the application of porcine models in translational research and clinical development.

Our assays are suitable for use with various porcine models, including the Hanford Minipig, Yucatan Minipig, Gottingen Minipig, Sinclair Minipig, and Yorkshire Pig. However, cross-reactivity may vary depending on the specific porcine model utilized in your research. For detailed information and to ensure the best fit for your study, please contact us or contact@evetechnologies.com.

With its targeted coverage, this assay provides reliable results from small sample volumes, making it a cost-effective choice for detailed cytokine profiling in porcine studies.

Click here for information on shipping biological samples for Discovery Assays    

 

Published Research Featuring Our PD13:
  1. Hefler, J., Hatami, S., Thiesen, A., Wagner, M. J., Mainardi, G., Himmat, S., Karvellas, C. J., Bigam, D. L., Freed, D. H., & Shapiro, A. M. J. (2024). Cyclosporine A does not mitigate liver ischemia/reperfusion injury in an ex vivo porcine model of donation after circulatory death. Annals of Transplantation, 29, e941054-1–e941054-13.  
  2. Inci, I., Arni, S., Iskender, I., Citak, N., Monné Rodriguez, J., Weisskopf, M., Opitz, I., Weder, W., Frauenfelder, T., Krafft, M. P., & Spahn, D. R. (2020). Functional, metabolic and morphologic results of ex vivo donor lung perfusion with a perfluorocarbon-based oxygen carrier nanoemulsion in a large animal transplantation model. Cells, 9(11), 2501. 
  3. Ehrsam, J. P., Arni, S., Weisskopf, M., Nowack, M., & Inci, I. (2023). Extracorporeal cytokine adsorption reduces systemic cytokine storm and improves graft function in lung transplantation. JTCVS Open, 15, 497-507. 
  4. Boston, T. E., Wang, F., Lin, X., Leonard, S., Kim, S. W., McKilligan, D., Fellner, V., & Odle, J. (2022). Gruel creep feeding accelerates growth and alters intestinal health of young pigs. Animals, 12(18), 2408.