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Human Angiogenesis & Growth Factor 17-Plex Discovery Assay® Array (HDAGP17)

$32.99

Biomarkers
Angiopoietin-2 | BMP-9 | EGF | Endoglin | Endothelin-1 | FGF-1 | FGF-2 | Follistatin | G-CSF | HB-EGF | HGF | IL-8 | Leptin | PLGF | VEGF-A | VEGF-C | VEGF-D

Price
$32.99/sample
(single analysis)

Sample Volume Requirements
Single analysis 75 μL
Duplicate analysis 100 μL  

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Species Type

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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type (Fee applies)

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout, fee applies)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions.) We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect. We typically recommend 2, one sample with a low expected analyte concentration and one with a high, to encompass the range of the standard curve.

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

Species:

Samples:

Tube Type:

Pilot Analysis:

Pilot Sample Numbers:

Infectious Nature:

Total Number of Samples:

**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Attention Clinicians! Our Long COVID Vascular Health 18-Plex Panel is now available for clinical diagnostics under CLIA regulation as a Laboratory Developed Test (LDT). This panel includes two simultaneous assays (HDAGP17 and HDSAA6) to assess vascular and endothelial integrity, coagulation, and inflammation—key aspects of long COVID. Running both assays together ensures efficient, high-quality results with a rapid turnaround. For details and to enhance your diagnostic capabilities, visit our patient diagnostics webpage. Reference intervals are available for PLASMA-EDTA samples.

Consider this assay for an extensive analysis of key factors involved in angiogenesis and growth regulation. The Human Angiogenesis & Growth Factor 17-Plex Discovery Assay® Array (HDAGP17) simultaneously measures Angiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1, FGF-2, Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PLGF, VEGF-A, VEGF-C, and VEGF-D.

This panel is ideal for exploring the complex interplay of growth factors and cytokines involved in vascular development, tissue repair, and disease states such as cancer and cardiovascular disorders. By evaluating these biomarkers, you can gain insights into processes like angiogenesis, inflammation, and cell proliferation.

Ideal for studying cancer progression, wound healing, and cardiovascular diseases, this assay provides insights into the roles of factors like VEGF-A and FGF-2 in angiogenesis and tissue regeneration. For instance, VEGF-A is crucial for new blood vessel formation, while FGF-2 promotes cell proliferation.

This assay offers a comprehensive tool for understanding the molecular mechanisms underlying pathological conditions and therapeutic responses.

Discover the power of precision with our assay, expertly designed for the Luminex® 200™ platform. This cutting-edge technology enables the simultaneous detection of multiple biomarkers from a minimal single sample, delivering comprehensive insights with unmatched efficiency. Streamline your research and unlock a new level of detail with our advanced multiplex assay solution.

Click here for information on shipping biological samples for Discovery Assays  

Published Research Featuring Our HDAGP17:
  1. Logan, S. M., & Storey, K. B. (2019). Angiogenic signaling in the lungs of a metabolically suppressed hibernating mammal (Ictidomys tridecemlineatus). PeerJ, 7, e8116. 
  2. Nguyen, K. T., Hecking, J., Berg, I. C., Kannappan, R., Donoghue, L., Ismail, E., Cheng, X., Giridharan, G. A., & Sethu, P. (2023). von Willebrand Factor and Angiopoietin-2 are sensitive biomarkers of pulsatility in continuous-flow ventricular assist device patients. ASAIO Journal, 69(6), 569-575.
  3. Haglund, T. A., Rajasekaran, N. S., Smood, B., Giridharan, G. A., Hoopes, C. W., Holman, W. L., Mauchley, D. C., Prabhu, S. D., Pamboukian, S. V., Tallaj, J. A., Rajapreyar, I. N., Kirklin, J. K., & Sethu, P. (2019). Evaluation of flow-modulation approaches in ventricular assist devices using an in-vitro endothelial cell culture model. The Journal of Heart and Lung Transplantation, 38(4), 456-465.
  4. Nguyen, K. T., Donoghue, L., Giridharan, G. A., Naber, J. P., Vincent, D., Fukamachi, K., Kotru, A., & Sethu, P. (2022). Acute response of human aortic endothelial cells to loss of pulsatility as seen during cardiopulmonary bypass. Cells Tissues Organs, 211(3), 324–334.