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Human Cytokine/Chemokine Panel A 48-Plex Discovery Assay® Array (HD48A)

$68.99

Biomarkers
sCD40L | EGF | Eotaxin | FGF-2 | Flt-3 ligand | Fractalkine | G-CSF | GM-CSF | GROα | IFNα2 | IFNγ | IL-1α | IL-1β | IL-1ra | IL-2 | IL-3 | IL-4 | IL-5 | IL-6 | IL-7 | IL-8 | IL-9 | IL-10 | IL-12p40 | IL-12p70 | IL-13 | IL-15 | IL-17A | IL-17E/IL-25 | IL-17F | IL-18 | IL-22 | IL-27 | IP-10 | MCP-1 | MCP-3 | M-CSF | MDC (CCL22) | MIG | MIP-1α | MIP-1β | PDGF-AA | PDGF-AB/BB | RANTES | TGFα | TNFα | TNFβ | VEGF-A

Price
$68.99/sample
(single analysis)

Sample Volume Requirements
Single analysis 75 uL

Duplicate analysis 100 uL

TO ORDER THIS ASSAY - DESIGN YOUR RESEARCH PROJECT BELOW

Species Type
Sample Type & Qty
Sample Tube Type
Pilot Analysis
Infectious Nature
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Sample Type & Qty

BAL Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Cell Lysate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

CSF

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Citrate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-EDTA

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Plasma-Heparin

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Serum

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Synovial Fluid

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue Homogenate

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Tissue/Cell Culture Supernatant

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Urine

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

Other

Sample

Number of Samples

Volume (uL)

Dilution Factor
This refers to the dilution factor of the samples that you are sending. Note that some assays recommend a dilution factor for assay performance or to reduce the demand on precious sample volume. For example, a number of rat and mouse cytokine arrays recommend a 2-fold dilution for serum/plasma samples (1 part sample and 1 part diluent) - please contact us for more information if needed.

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Sample Tube Type
Conforming tubes are 0.5-0.65mL snap cap tubes. Having a conforming tube helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample tubes are non-conforming.

0.5-0.65ml Snap Cap

Other Tube Type

Label Type
Conforming tube labeling is with a constant letter from your last name followed by consecutive numbers. (ie. Last name Smith: S1, S2, S3, etc.). Labelling tubes in this manner helps us complete your analysis efficiently and accurately. A $4.00 charge per sample tube may be added to your order if sample labelling is non-conforming.

Alpha Numeric Labeling (1 letter with consecutive numbers, i.e. A1,A2, A3, etc.)

Other Label Type (Attach sample list at checkout)

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Pilot Analysis

Pilot runs are recommended for medium to large cohorts of samples that lack homogeneity (i.e. n>20 of tissue homogenate samples, cell culture samples, or less common sample types which can have higher sources of variability and/or unpredictable analyte levels). Running a pilot in such instances will ensure an optimal dilution factor is used before running your entire sample cohort. Pilot runs are ideally done on samples that reflect the expected experimental range and are provided as separate aliquots/separate samples designated for the pilot run.

None

Yes - I will include additional pilot samples (RECOMMENDED: to remove consideration for inter-assay variables between pilot and main sample cohort assay sessions)

Yes – I will indicate pilot samples from within my cohort (CAUTION: pilot samples will not be run again with the main assay. Inter-assay variables may result in a batch effect)

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Infectious Nature
If your samples are of an infectious nature, please contact us PRIOR TO SHIPPING. Please DO NOT SHIP infectious samples unless you have contacted us for requirements.

No

Yes (please state the infectious nature for reivew prior to shipping)

Review
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Review

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Samples:

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Infectious Nature:

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**Select the Replication in Which You Want Your Samples Tested:

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Description

This assay uses the Luminex® 200™ platform.

Consider our most popular human array, the Human Cytokine/Chemokine Panel A 48-Plex Discovery Assay® Array (HD48A), for a thorough analysis of 48 human cytokines and chemokines simultaneously from a small sample volume. This powerful assay includes essential biomarkers such as IL-6, TNFα, VEGF-A, and IL-10, providing comprehensive coverage of immune and inflammatory responses.

The HD48A panel is exceptionally valuable for researching a diverse array of conditions, including cancer, cardiovascular diseases, and autoimmune disorders. It delivers an in-depth view of the circulating immune microenvironment in both acute and chronic inflammatory conditions, as well as autoimmune and autoinflammatory diseases.

For example, the panel can illuminate critical biomarkers like IL-6 and TNFα, which are pivotal in chronic inflammatory diseases such as rheumatoid arthritis and lupus. It also measures VEGF-A, a key player in tumor progression and angiogenesis. This rich dataset supports a deeper understanding of disease mechanisms and enhances your research into complex inflammatory and immune responses.

Consider using the Human Cytokine/Chemokine 96-Plex Discovery Assay® Array (HD96) for your analysis. This advanced array allows the simultaneous execution of two assays—HD48A and HD48B—from a single small sample volume, offering both efficiency and the most comprehensive cytokine and chemokine profile available.

If you anticipate that your samples may have low cytokine levels, such as those from normal healthy populations, you might want to consider our High Sensitivity panel. This advanced option is specifically designed to detect low abundance cytokines with greater precision, ensuring you capture even subtle variations in your data.

Discover the power of precision with our assay, expertly designed for the Luminex® 200™ platform. This cutting-edge technology enables the simultaneous detection of multiple biomarkers from a minimal single sample, delivering comprehensive insights with unmatched efficiency. Streamline your research and unlock a new level of detail with our advanced multiplex assay solution.

Recommended Dilution: Most samples are run undiluted, however RANTES has high concentrations in serum and plasma. If this target is important to your study and your sample type is serum or plasma, it is recommended to run samples in a separate assay with a dilution factor applied or utilize our Human Supplemental Biomarker 10-Plex (HDHSB10)

If you expect your samples to have low cytokine levels, i.e. normal healthy populations, you may wish to inquire about a High Sensitivity panel.

Click here for information on shipping biological samples for Discovery Assays

 

Published Research Featuring Our HD48A:
  1. PNAS! Halasz, H., Malekos, E., Covarrubias, S., +8 additional authors, & Carpenter, S. (2024). CRISPRi screens identify the lncRNA, LOUP, as a multifunctional locus regulating macrophage differentiation and inflammatory signaling. Proceedings of the National Academy of Sciences, 121(22), e2322524121.
  2. Gomes, A. C., Sousa, D. M., Oliveira, T. C., Fonseca, Ó., Pinto, R. J., Silvério, D., Fernandes, A. I., Moreira, A. C., Silva, T., Teles, M. J., Pereira, L., Saraiva, M., & Lamghari, M. (2023). Serum amyloid A proteins reduce bone mass during mycobacterial infections. Frontiers in Immunology, 14.
  3. Halasz, H., Malekos, E., Covarrubias, S., Yitiz, S., Montano, C., Sudek, L., Katzman, S., Liu, S. J., Holbeck, M. A., Weissman, J. S., & Carpenter, S. (2023). CRISPRi screens identify the lncRNA, LOUP, as a multifunctional locus regulating macrophage differentiation epigenetically and inflammatory signaling through a short, encoded peptide. bioRxiv.